产品货号：
GS4854
中文名称：
重组PreScission酶
英文名称：
Recombinant PreScission Protease
产品规格：
100IU
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
概述
PreScission protease is a cysteine protease derived from human rhinovirus – HRV3C Protease.rPP is a fusion protein of glutathione S-transferase(GST)and human rhinovirus(HRV)type 14 3C protease.It specifically recognizes the amino acid sequences which include the core site of Leu-Phe-Gln-Gly-Pro and cleaves between the Gln and Gly residues.Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals,but also upon the super structures of the fusion protein.rPP works most effective at 4℃ and can digest substrates at room temperature as well.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE
技术规格
相关搜索：重组PreScission酶，PPase, Recombinant，Recombinant PreScission Protease
概述
PreScission protease is a cysteine protease derived from human rhinovirus – HRV3C Protease.rPP is a fusion protein of glutathione S-transferase(GST)and human rhinovirus(HRV)type 14 3C protease.It specifically recognizes the amino acid sequences which include the core site of Leu-Phe-Gln-Gly-Pro and cleaves between the Gln and Gly residues.Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals,but also upon the super structures of the fusion protein.rPP works most effective at 4℃ and can digest substrates at room temperature as well.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE
技术规格
| 外观 | Sterile colorless liquid. |
| 来源 | Escherichia coli. |
| Recommended Conditions for Cleavage of a Fusion Protein | During cleavage reactions,it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield,purity,and extent of digestion.The amount of PreScission Protease,temperature and length of incubation required |
| Cleavage Buffer | 50mMTris-HCl,pH 7.0(at 25℃),150mMNaCl,1 mM EDTA,1 mM dithiothreitol.Chill to 5 ℃ prior to use. |
| Unit Definition | One unit is defined as the amount of enzyme needed to cleave 100μgof fusion protein in 16hoursto 90%completion at 5 ℃ in a buffer containing 50mMTris-HCl,pH 7.0,150mMNaCl,1 mM EDTA,and 1 mM DTT. |
相关搜索：重组PreScission酶，PPase, Recombinant，Recombinant PreScission Protease